Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
June 1, 1991Journal of VirologyOpen Access

Genetic analysis of porcine respiratory coronavirus, an attenuated variant of transmissible gastroenteritis virus

View Full Paper
Ask AI
Bookmark
Share

Key result

Porcine respiratory coronavirus (PRCV) differs from transmissible gastroenteritis virus (TGEV) by a 681-nucleotide deletion in the spike gene and a point mutation that eliminates mRNA 3.

Population

Swine testicular cells infected with porcine respiratory coronavirus and transmissible gastroenteritis virus

Comparison

Genetic analysis vs Classical enterotropic transmissible…

Design

Preclinical

Authors

RWRonald D. WesleyRWRoger D. WoodsACAndrew Cheung

Discussion

Loading...

Member takes

Overview

Distinguishes PRCV from TGEV genetically; leaves open functional roles in tropism and intervention strategies.

Structured PICO

P
Population
Swine testicular (ST) cells infected with porcine respiratory coronavirus (PRCV-Ind/89) and transmissible gastroenteritis virus (TGEV-PP3)
E
Exposure
Genetic analysis (Northern blotting, S1 nuclease mapping, nucleotide sequencing)
C
Comparator
Classical enterotropic transmissible gastroenteritis virus (TGEV-PP3)
O
Outcome
Genome and transcriptional pattern differencessurrogate

The attenuated respiratory variant of TGEV (PRCV) contains specific deletions in the S gene and mutations eliminating mRNA 3, which likely explain its altered tissue tropism and reduced virulence.

Limitations

  • It is not known whether the smaller 7.5-kb RNA 2(S) of PRCV is sufficient to account for the more rapid migration of the PRCV RNA 1 species or whether additional deletions might be present.
  • It cannot be ascertained whether the deletion in the S gene, the lack of mRNA 3, or other genetic differences altered the tropism of PRCV-Ind/89 for enteric enterocytes.

Cite This Study

Wesley et al. (1991) studied Porcine respiratory coronavirus (PRCV). Porcine respiratory coronavirus (PRCV-Ind/89) vs. Transmissible gastroenteritis virus (TGEV-PP3) was evaluated on Genetic structure and transcriptional pattern differences. Porcine respiratory coronavirus (PRCV) differs from transmissible gastroenteritis virus (TGEV) by a 681-nucleotide deletion in the spike gene and a point mutation that eliminates mRNA 3.

synapsesocial.com/papers/6a6e16c3a528af2d65c2b2a3https://doi.org/10.1128/jvi.65.6.3369-3373.1991
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1The Polypeptide of Mr 14000 of Porcine Transmissible Gastroenteritis Virus: Gene Assignment and Intracellular Location1989 · 24 citations
  2. 2Genetic basis for the pathogenesis of transmissible gastroenteritis virus1990 · 56 citations
  3. 3Coronavirus subgenomic minus-strand RNAs and the potential for mRNA replicons.1989 · 250 citations
  4. 4Minus-strand copies of replicating coronavirus mRNAs contain antileaders1991 · 167 citations