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Working with 14CH3-labeled 2'-O-methyladenosine, it has been shown that the ether linkage is cleaved during digestion with 70% perchloric acid at 100° for 25 min, yielding a volatile product, methanol. The heterocyclic base residues of nucleosides and their methyl groups have been shown to be relatively stable to this treatment. This procedure has been used to examine the degree of sugar methylation in the transfer RNA of an Escherichia coli strain and the result agrees well with values from this and other laboratories obtained by more laborious procedures.
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Baskin et al. (1967) studied this question.
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