What question did this study set out to answer?

To design and construct an automated mechanical puller for reliably manufacturing uniform glass microelectrodes with tip diameters below 0.5 µm for intracellular recordings.

July 1, 1953Review of Scientific Instruments

An Instrument for the Production of Microelectrodes Used in Electrophysiological Studies

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Authors

JAJ. T. AlexanderColumbia University WNWilliam L. NastukColumbia University

Discussion

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Implication

Technical report demonstrates automated fabrication of sub-half-micron glass microelectrodes, indicating improved consistency for intracellular electrophysiology.

Key Points

To design and construct an automated mechanical puller for reliably manufacturing uniform glass microelectrodes with tip diameters below 0.5 µm for intracellular recordings.
A 35-mm section of Pyrex tubing (outside diameter 2 mm, inside diameter 1.3 mm) is surrounded and heated by an electrically energized platinum coil.
A solenoid and plunger apply an initial 100-g longitudinal pulling force until the softened tubing elongates by 5 mm, triggering an automatic 1700-g pull that separates the glass into two micropipettes.
The automated puller routinely produces micropipette tip diameters under 0.5 µm suitable for measuring single muscle fiber membrane potentials.
Standardized fabrication minimizes manual variability, producing consistent tip geometry and predictable electrolytic resistance profiles.

Cite This Study

Alexander et al. (1953) studied this question.

synapsesocial.com/papers/6a6ed4b435aa2c282ce06943 https://doi.org/10.1063/1.1770777

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July 1, 1953Review of Scientific Instruments

An Instrument for the Production of Microelectrodes Used in Electrophysiological Studies

View Full Paper

Ask AI

Bookmark

Authors

JAJ. T. AlexanderColumbia University WNWilliam L. NastukColumbia University

Discussion

Member takes

Implication

Technical report demonstrates automated fabrication of sub-half-micron glass microelectrodes, indicating improved consistency for intracellular electrophysiology.

Key Points

To design and construct an automated mechanical puller for reliably manufacturing uniform glass microelectrodes with tip diameters below 0.5 µm for intracellular recordings.
A 35-mm section of Pyrex tubing (outside diameter 2 mm, inside diameter 1.3 mm) is surrounded and heated by an electrically energized platinum coil.
A solenoid and plunger apply an initial 100-g longitudinal pulling force until the softened tubing elongates by 5 mm, triggering an automatic 1700-g pull that separates the glass into two micropipettes.
The automated puller routinely produces micropipette tip diameters under 0.5 µm suitable for measuring single muscle fiber membrane potentials.
Standardized fabrication minimizes manual variability, producing consistent tip geometry and predictable electrolytic resistance profiles.

Cite This Study

Alexander et al. (1953) studied this question.

synapsesocial.com/papers/6a6ed4b435aa2c282ce06943 https://doi.org/10.1063/1.1770777

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