Amelioration effect of 18β-Glycyrrhetinic acid on methylation inhibitors in hepatocarcinogenesis -induced by diethylnitrosamine

Figures

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  Figure 1. Chemical induction of HCC in albino rats treated with different effectors. (A) Body weight after treatment of HCC with different effectors. (B) Organ weight (lung, heart, kidney and liver) after treatment of HCC with different effectors.

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  Figure 2. Histological studies on evaluation of different compounds against HCC induced in albino rats A photomicrographic section of normal liver from the negative control group clearly shows normal liver morphology, whereas the positive control carcinogenic group demonstrates necrobiotic alterations and focal pleomorphic neoplastic cells, and rat liver treated with DOX illustrates mild swelling of hepatic carcinoma cells with hyperplasia of Kupffer cells. Poor <50% tumor necrosis, but with rat liver treated with GA showing necrobiotic changes of hepatic carcinoma cells with 51%-99% tumor necrosis, while also rat liver treated with GA and DOX showing small focal necrotic areas with apoptosis of a few hepatocytes, rat liver treated with bacteria showing vacuolar degeneration of hepatic carcinoma cells with hyperplasia of Kupffer cells and poor <50%percent tumor necrosis, and photomicrograph of rat liver section demonstrated necrobiotic changes of hepatic carcinoma cells. (A) Negative control animal. (B) positive control animals (rats with HCC). (C) The animal administrated received GA (100 mg/kg. orally) daily for 4 weeks. (D) Rats were injected with Doxorubicin (50 mg/kg) daily by i.p for…

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  Figure 3. Measurement of the cofactor of methylation activity (DNMT1and MS) by qRT-PCR and flow cytometery. Positive control increased DNMT1 to 7-fold change while other treatment makes significant downregulation (A). Error bars indicate standard deviation of three independent experiments. The student’s two-tailed t-test was used for statistical analysis. P-value ≤ 0.05 was considered statistically significant. Positive control increased MS to 10-fold change while other treatment makes significant downregulation (B). Protein expression profile of DNMT1 and MS indicated by red and blue dotes using secondary antibodies Alex Flour-488 and 594, respectively (C). *statistically significant difference as compared with the controls (P, 0.05 for each). While, ** statistically significant difference as compared with the controls (P, 0.01 for each).

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  Figure 4. Methylation activity based on relative methylation in TET1 and DLC-1 in coding sequence and by restriction enzyme on the promoter region. (A) Fold change of methylated activity of TET-1 based on coding sequence. (B) Agarose gel electrophoresis of TET-1 gene segment that digested with CpG restriction enzyme HpaII indicates all derived samples in rats treated with GA, DOX, a combination of GA with bacteria and combination of GA with DOX in addition to controlling samples. (C) The methylation activity of DLC-1 is indicated by methylation fold change. The error bar indicates the stander deviation between two different replicates; statically, the student’s two-tails t-test test has been differentiating significantly. (D) Agarose gel electrophoresis of DLC-1 gene segment that digested with CpG restriction enzyme HpaII indicated all derived samples in rats treated with GA, DOX, a combination of GA with bacteria, and a combination of GA with DOX in addition to control samples. *statistically significant difference as compared with the controls (P, 0.05 for each). While, ** statistically significant difference as compared with the controls (P, 0.01 for each).

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  Figure 5. Relative methylation activity of NF-Kb and STAT3 based on RNA expression. (A, C) Relative methylation activity NF-kB and STAT-3 gene sequence indicated by fold change that significantly increased upon GA treatment and its combination with DOX or the bacteria. (B, D) relative expression of NF-kB and STAT-3 genes significantly reduced upon GA treatment and its indicated combination. Protein expression profile of NF-kB and STAT3 indicated by red and blue dotes using secondary antibodies Alex Flour-488 and 594, respectively (E). *statistically significant difference as compared with the controls (P, 0.05 for each). While, ** statistically significant difference as compared with the controls (P, 0.01 for each).

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  Figure 6. Relative expression of TET-1 and DLC-1 gene expression in GA and combination of GA with bacteria-treated rats. (A, B) Relative gene expression of TET-1 and DLC-1 indicated by fold change that was subjected to GA and combination of GA with Bacteria (lactobacillus rhamanosus) comparison with control. (C) Quantification of protein profile of TET-1 and DLC-1 in treated rats indicated by flow cytometry. *statistically significant difference as compared with the controls (P, 0.05 for each). While, ** statistically significant difference as compared with the controls (P, 0.01 for each).