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The aim is to isolate and characterize lipase-producing bacteria from petroleum-contaminated soil for enzyme production and bioremediation.

August 2, 2026Applied MicrobiologyOpen Access

Lipase Production and Characterization from Serratia liquefaciens Isolated from Petroleum-Contaminated Soil

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Randomized trial reveals lipase production capabilities in Serratia liquefaciens, suggesting potential for environmental cleanup.

The aim is to isolate and characterize lipase-producing bacteria from petroleum-contaminated soil for enzyme production and bioremediation.
Lipase-producing bacteria were isolated from petroleum-contaminated soil and characterized.
Various inducer oils and agro-industrial residues were assessed for enhancing enzyme production.
Kinetic and stability assays were conducted to evaluate the properties of purified lipase AB1.
The enzyme lipase AB1 exhibited a specific activity of 610.92 U/mg with a 9.42-fold purification increase and 76% recovery.
Optimal catalytic activity was achieved at 60 °C and pH 8, with stability for up to 120 min.
Kinetic analysis showed a Km value of 30 µM and Vmax of 23.89 U/mL, indicating high efficiency.

Baruwa et al. (2026) studied this question.

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