What question did this study set out to answer?

To develop and evaluate Digital Genotyping, a restriction enzyme-targeted resequencing method for high-throughput, cost-effective genetic analysis in species with large, repeat-rich genomes.

July 5, 2013BMC GenomicsOpen Access

Digital genotyping of sorghum – a diverse plant species with a large repeat-rich genome

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Authors

DMDaryl T. MorishigeTexas A&M University PKPatricia E. KleinThe University of Texas at Austin JHJosie HilleyTexas A&M University

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Implication

Genomic study demonstrates efficient targeted resequencing across repeat-rich genomes in sorghum, highlighting a cost-effective approach for high-resolution genetic mapping and QTL analysis.

Key Points

To develop and evaluate Digital Genotyping, a restriction enzyme-targeted resequencing method for high-throughput, cost-effective genetic analysis in species with large, repeat-rich genomes.
Generated sequencing templates using three methylation-sensitive restriction enzymes recognizing nested 4, 6, or 8 bp GC-rich sequences.
Constructed a high-density genetic map using 1,772 markers across 137 recombinant inbred lines.
Acquired the expected allele sequence 97.3% of the time, achieving an expected-to-alternative allele sequence ratio greater than 20:1.
Generated a genetic map aligned to the reference genome at an average resolution of 1.47 cM, which refined plant height QTL mapping to specific loci such as Dw3.
Identified correct placements for two misassembled genomic regions and assigned positions to seven unmapped super contigs in the sorghum reference genome.

Cite This Study

Morishige et al. (2013) studied this question.

synapsesocial.com/papers/6a6f12da8031ec7bb1db948d https://doi.org/10.1186/1471-2164-14-448

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Implication

Genomic study demonstrates efficient targeted resequencing across repeat-rich genomes in sorghum, highlighting a cost-effective approach for high-resolution genetic mapping and QTL analysis.

Digital genotyping of sorghum – a diverse plant species with a large repeat-rich genome

Discussion

Member takes

Implication

Key Points

Cite This Study

Digital genotyping of sorghum – a diverse plant species with a large repeat-rich genome

Discussion

Member takes

Implication

Key Points

Cite This Study

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