Key result
Urine testing for JEV RNA failed to detect the virus in any of 41 patients with acute encephalitis syndrome, demonstrating that viral RNA degradation makes urine a challenging diagnostic specimen.
Why the study?
JEV diagnosis relies on difficult-to-interpret serology because viral RNA is rarely detected in serum or CSF, and testing urine feasibility had minimal prior investigation.
Does the use of lysis buffer improve the detection of JEV RNA in urine samples from patients with suspected CNS infections?
Population
41 patients with acute encephalitis syndrome admitted to the hospital in Laos
Comparison
Urine storage with lysis buffer and a Microsep device vs without
Design
Two studies evaluating consecutive hospital-admitted patients
Authors
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Urine should not be used for JEV RNA detection in encephalitis; leaves open whether lysis buffer improves yield in future studies.
Observational (n=41)
Does the use of lysis buffer improve the detection of JEV RNA in urine samples from patients with suspected CNS infections?
Viral RNA degradation makes urine a challenging specimen for JEV detection, though immediate addition of lysis buffer may improve detection limits.
Bharucha et al. (2019) conducted an observational in Acute encephalitis syndrome / suspected CNS infection (n=41). Urine testing for JEV RNA was evaluated on Detection of JEV RNA in urine. Urine testing for JEV RNA failed to detect the virus in any of 41 patients with acute encephalitis syndrome, demonstrating that viral RNA degradation makes urine a challenging diagnostic specimen.
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