What question did this study set out to answer?

To identify the molecular and matrix requirements that allow fibroblasts to transmigrate from collagenous connective tissue into a provisional fibrin clot during early wound healing.

April 1, 1997Journal of Cell Science

Fibronectin provides a conduit for fibroblast transmigration from collagenous stroma into fibrin clot provisional matrix

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Authors

DGDoris Greiling RCRichard A.F. ClarkSanford USD Medical Center

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Implication

In vitro investigation demonstrates fibronectin-dependent fibroblast invasion from collagen into provisional fibrin matrices, highlighting critical cellular pathways in early wound repair.

Key Points

To identify the molecular and matrix requirements that allow fibroblasts to transmigrate from collagenous connective tissue into a provisional fibrin clot during early wound healing.
Established a 3D in vitro model simulating fibroblast migration across the interface between a collagen gel and a fibrin clot.
Tested cell migration following stimulation with platelet releasate or PDGF-BB, after gamma irradiation, and in the presence of serine protease inhibitors.
Assessed transmigration dependence on matrix fibronectin content and evaluated inhibition by RGD peptides and monoclonal antibodies against alpha5 beta1 and alpha v beta3 integrins.
PDGF-BB and platelet releasate induced fibroblast transmigration in a concentration-dependent manner, functioning independently of cell proliferation.
Serine protease inhibition blocked fibrin clot invasion without impairing exit from the collagen gel, whereas fibronectin depletion from either gel markedly reduced transmigration.
Transmigration was significantly inhibited by RGD peptides and by blocking antibodies directed against the alpha5 beta1 and alpha v beta3 integrin receptor subunits.

Cite This Study

Greiling et al. (1997) studied this question.

synapsesocial.com/papers/6a6f1533f44fa9f079dc78ed https://doi.org/10.1242/jcs.110.7.861

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April 1, 1997Journal of Cell Science

Fibronectin provides a conduit for fibroblast transmigration from collagenous stroma into fibrin clot provisional matrix

View Full Paper

Ask AI

Bookmark

Authors

DGDoris Greiling RCRichard A.F. ClarkSanford USD Medical Center

Discussion

Member takes

Implication

In vitro investigation demonstrates fibronectin-dependent fibroblast invasion from collagen into provisional fibrin matrices, highlighting critical cellular pathways in early wound repair.

Key Points

To identify the molecular and matrix requirements that allow fibroblasts to transmigrate from collagenous connective tissue into a provisional fibrin clot during early wound healing.
Established a 3D in vitro model simulating fibroblast migration across the interface between a collagen gel and a fibrin clot.
Tested cell migration following stimulation with platelet releasate or PDGF-BB, after gamma irradiation, and in the presence of serine protease inhibitors.
Assessed transmigration dependence on matrix fibronectin content and evaluated inhibition by RGD peptides and monoclonal antibodies against alpha5 beta1 and alpha v beta3 integrins.
PDGF-BB and platelet releasate induced fibroblast transmigration in a concentration-dependent manner, functioning independently of cell proliferation.
Serine protease inhibition blocked fibrin clot invasion without impairing exit from the collagen gel, whereas fibronectin depletion from either gel markedly reduced transmigration.
Transmigration was significantly inhibited by RGD peptides and by blocking antibodies directed against the alpha5 beta1 and alpha v beta3 integrin receptor subunits.

Cite This Study

Greiling et al. (1997) studied this question.

synapsesocial.com/papers/6a6f1533f44fa9f079dc78ed https://doi.org/10.1242/jcs.110.7.861

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