What question did this study set out to answer?

To identify and characterize transient intermediate stages involved in transport vesicle budding and membrane fusion within a cell-free Golgi transport system.

February 1, 1986Journal of Biological ChemistryOpen Access

A novel prefusion complex formed during protein transport between Golgi cisternae in a cell-free system.

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Authors

BWBinks W. WattenbergVirginia Commonwealth University WBWilliam E. BalchNorthwestern University JRJames E. RothmanYale University

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Implication

In vitro biochemical study reveals a dilution-resistant prefusion intermediate in Golgi transport, indicating multi-step vesicle maturation before membrane fusion.

Key Points

To identify and characterize transient intermediate stages involved in transport vesicle budding and membrane fusion within a cell-free Golgi transport system.
Reconstituted intercompartmental protein transport between isolated Golgi cisternae in a cell-free assay.
Assessed intermediate stages by measuring membrane fusion kinetics under varying cytosol concentrations, donor-acceptor membrane combinations, and sensitivity to N-ethylmaleimide (NEM) and ATP.
Identified a novel dilution-resistant transport intermediate that arises strictly in the concurrent presence of both donor and acceptor membranes.
Demonstrated that consumption of the dilution-resistant intermediate requires substantially less cytosol than its initial assembly and remains susceptible to NEM inhibition.
Found that progression from this intermediate to the NEM-resistant prefusion state requires both ATP and cytosol, marking a distinct pre-fusion vesicle processing step.

Cite This Study

Wattenberg et al. (1986) studied this question.

synapsesocial.com/papers/6a6f17d626a7f98052dbd7f5 https://doi.org/10.1016/s0021-9258(17)35918-5

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Implication

In vitro biochemical study reveals a dilution-resistant prefusion intermediate in Golgi transport, indicating multi-step vesicle maturation before membrane fusion.

A novel prefusion complex formed during protein transport between Golgi cisternae in a cell-free system.

Discussion

Member takes

Implication

Key Points

Cite This Study

A novel prefusion complex formed during protein transport between Golgi cisternae in a cell-free system.

Discussion

Member takes

Implication

Key Points

Cite This Study

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