Experimental study demonstrates rapid modification of carboxyl groups in proteins using carbodiimides, suggesting broad utility for structure-function analyses.
A method for the rapid modification under mild conditions of carboxyl groups in proteins has been developed. The method involves the activation of the carboxyl group by a water-soluble carbodiimide and the subsequent reaction of the activated carboxyl group with a nucleophile such as glycine methyl ester. The kinetics of the reaction indicates that there are limits to the variation of carbodiimide and nucleophile when quantitative modification is desired, but that a wide variety of reagents can be used if quantitative reaction is not essential. Thus, variation in the structure of the carbodiimide can affect the carboxyl groups activated, and variation in the charge, size, and chemical and spectral properties of the nucleophile can alter the type of modification at a specific carboxyl group. The reaction proceeds equally well in high concentrations of urea or guanidine hydrochloride. The method appears to be of wide utility for analytical purposes and structure-function correlations.
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Hoare et al. (1967) studied this question.
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