A series of classic papersl-5 has described the discovery and many of the properties of a specific transport system associated with the function of the lac operon in Escherichia coli.In 1965, a working model for the lactose transport system was proposed6 in which a membrane-localized protein (M protein) plays a central role.This protein, recognized on the basis of its sensitivity to NEM and its affinity for
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Scarborough et al. (1968) studied this question.