What question did this study set out to answer?

To identify the specific morphological cell types in rat taste buds that express key phospholipase C signaling components and examine their structural interactions with nerve fibers.

December 4, 2003The Journal of Comparative Neurology

Morphologic characterization of rat taste receptor cells that express components of the phospholipase C signaling pathway

View Full Paper

Ask AI

Bookmark

Authors

TCTod R. ClappColorado State University RYRuibiao YangChinese Academy of Sciences CSCristi L. StoickUniversity of Washington

Discussion

Member takes

Implication

Ultrastructural analysis reveals IP3R3 expression predominantly in rat type II taste cells lacking typical synapses, suggesting unconventional communication between receptor cells and nerve fibers.

Key Points

To identify the specific morphological cell types in rat taste buds that express key phospholipase C signaling components and examine their structural interactions with nerve fibers.
Examined rat taste buds using 3,3'-diaminobenzidine tetrahydrochloride immunoelectron microscopy and fluorescence microscopy to detect type III IP(3) receptor (IP3R3) expression.
Performed confocal microscopy to compare IP3R3 and PLCbeta2 immunoreactivity with established cell markers, including serotonin, protein gene-regulated product 9.5, and neural cell adhesion molecule.
Cytoplasmic IP3R3 immunoreactivity localized to a large subset of type II taste cells and a minor subset of type III cells.
Type II taste cells lacked conventional synapses with nerve fibers but exhibited subsurface cisternae of smooth endoplasmic reticulum at close membrane appositions with adjacent nerve processes.

Cite This Study

Clapp et al. (2003) studied this question.

synapsesocial.com/papers/6a6f1e7e6c240de38cdb647f https://doi.org/10.1002/cne.10963

View Full Paper

Ask AI

Bookmark

Implication

Ultrastructural analysis reveals IP3R3 expression predominantly in rat type II taste cells lacking typical synapses, suggesting unconventional communication between receptor cells and nerve fibers.

Morphologic characterization of rat taste receptor cells that express components of the phospholipase C signaling pathway

Discussion

Member takes

Implication

Key Points

Cite This Study

Morphologic characterization of rat taste receptor cells that express components of the phospholipase C signaling pathway

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider