Key result
The isolated infectious bursal disease virus strain HB202201 belongs to the novel variant A2dB1 genotype and possesses multiple specific amino acid mutations and altered antigenic regions.
Why the study?
Current vaccines do not provide complete protection against emerging novel variant IBDV strains in China, and a lack of comprehensive genomic analysis has hampered vaccine development.
Genomic analysis of the HB202201 IBDV strain reveals it belongs to the A2dB1 genotype with specific mutations, providing insights for novel vaccine development against variant IBDV in chickens.
The infectious bursal disease virus (IBDV) is a member of the viruses that can induce immunosuppression in chickens. In recent years, more and more IBDV-infected cases by the novel variant IBDV were reported in China, and it has been demonstrated that currently used vaccines could not provide complete protection against these new IBDV variants. However, a lack of comprehensive analysis of the genomic characteristics of the novel variant strain IBDV has hampered its vaccine development. In this study, a strain of IBDV, designated HB202201, was phylogenetically analyzed, and it was found that the hypervariable region (HVR) of VP2 belonged to the novel variant strain. Furthermore, the 5'- and 3'-ends of segments A and B were analyzed using the rapid amplification of cDNA end (RACE) method. After the full-length of segment A and segment B were determined, the phylogenetic analysis of the segment A and segment B showed that the isolated HB202201 belonged to A2dB1 genotype, which demonstrated the HB202201 belonged to the novel variant strain. In addition, the specific mutations in VP1-VP5 amino acids were analyzed, which showed that there were multiple typical mutations in novel variant IBDV proteins, including VP1 (G24, I141, V163, and E240), VP2 (K221, and I252), VP3 (Q167 and L196), and VP5 (R7, P44, R92, G104, and E147), whereas there was no typical mutation in VP4. This study provides insights into the genomic and antigenic characteristics of the novel variant IBDV, which will promote the development of novel vaccine against the novel variant IBDV.
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Bo et al. (2023) studied Infectious bursal disease virus (IBDV). HB202201 strain was evaluated on Genomic and antigenic characteristics. The isolated infectious bursal disease virus strain HB202201 belongs to the novel variant A2dB1 genotype and possesses multiple specific amino acid mutations and altered antigenic regions.
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