ICSH recommendations for the measurement of Haemoglobin A₂

Summary Although DNA analysis is needed for characterization of the mutations that cause β‐thalassaemia, measurement of the Hb A 2 is essential for the routine identification of people who are carriers of β‐thalassaemia. The methods of quantitating Hb A 2 are described together with pitfalls in undertaking these laboratory tests with particular emphasis on automated high‐performance liquid chromatography and capillary electrophoresis.