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One-pot synthesis: A system has been developed to glycosylate peptides, proteins, and oligosaccharides more efficiently than conventional procedures. With the simple precursors GalNAc, UTP, and creatine-P, four enzymes are employed in one pot to regenerate UDP-GalNAc which is used for the transfer of GalNAc to specific acceptor substrates.
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Bourgeaux et al. (2006) studied this question.
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