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March 1, 1999Microbiology

Porin expression in clinical isolates of Klebsiella pneumoniae

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Authors

SHSantiago Hernández-AllésMediterranean Institute for Advanced StudiesSASebastián AlbertíHealth Research Institute of the Balearic IslandsDÁDolores Vargas ÁlvarezUniversidad Autónoma de Guerrero

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Implication

Laboratory study reveals loss of OmpK35 porin expression in beta-lactamase-producing clinical isolates of Klebsiella pneumoniae, indicating a mechanism of enhanced antimicrobial resistance.

Key Points

  • To optimize gel electrophoresis techniques for distinguishing Klebsiella pneumoniae porins OmpK35 and OmpK36 and examine their expression across clinical isolates producing extended-spectrum beta-lactamases.
  • Modified the bisacrylamide content in polyacrylamide resolving gels to improve separation of OmpK35 and OmpK36.
  • Cultured isolates in low-osmolarity media to stimulate OmpK35 expression and paired migration profiles with Western blot analysis for definitive porin identification.
  • Compared outer membrane porin expression profiles between clinical isolates producing extended-spectrum beta-lactamases and non-producing isolates.
  • Electrophoretic migration order between OmpK35 and OmpK36 inverted across different strains, preventing reliable identification by apparent molecular mass alone without osmolarity manipulation and Western blotting.
  • Isolates lacking extended-spectrum beta-lactamases consistently expressed both OmpK35 and OmpK36 porins.
  • Isolates producing extended-spectrum beta-lactamases predominantly expressed only OmpK36, showing either undetectable or severely depleted levels of OmpK35.

Cite This Study

Hernández-Allés et al. (1999) studied this question.

synapsesocial.com/papers/6a6f29bc75498292b7088edfhttps://doi.org/10.1099/13500872-145-3-673
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Electrophoretic resolution of the ‘major outer membrane protein’ of <i>Escherichia coli</i> K12 into four bands1975 · 1,419 citations
  2. 2Sensitivity of Escherichia coli to various β‐lactams is determined by the interplay of outer membrane permeability and degradation by periplasmic β‐lactamases: a quantitative predictive treatment1987 · 185 citations
  3. 3Cross-Resistance to Nalidixic Acid, Trimethoprim, and Chloramphenicol Associated with Alterations in Outer Membrane Proteins of Klebsiella, Enterobacter, and Serratia1985 · 191 citations
  4. 4Loss of OmpC Porin in a Strain of Salmonella typhimurium Causes Increased Resistance to Cephalosporins During Therapy1987 · 77 citations
  5. 5Repression of porin synthesis by salicylate inEscherichia coli, Klebsiella pneumoniaeandSerratia marcescens1987 · 47 citations