Key result
Hepatic OSBP overexpression increases VLDL and liver triglycerides, raising triglyceride secretion rate ~70% in mice.
Demonstrates that oxysterol binding protein increases hepatic triglyceride synthesis and secretion in mice, suggesting a role in insulin-regulated lipogenesis.
BACKGROUND: Oxysterol binding protein (OSBP) has previously been implicated as a sterol sensor that regulates sphingomyelin synthesis and the activity of extracellular signal-regulated kinases (ERK). METHODS AND RESULTS: We determined the effects of adenovirus-mediated hepatic overexpression of OSBP and its homologues ORP1L and ORP3 on mouse serum lipids. Whereas ORP1L and ORP3 had no effect on serum lipids, OSBP induced a marked increase of VLDL triglycerides (TG). Also, the liver tissue TG were elevated in the AdOSBP-injected mice, and their TG secretion rate was increased by 70%. The messenger RNAs for enzymes of fatty acid synthesis and their transcriptional regulator, SREBP-1c, as well as the Insig-1 mRNA, were upregulated two-fold in the OSBP-expressing livers. No change occurred in the messages of liver X receptor target genes ABCA1, ABCG5, and CYP7A1, and the Insig-2a mRNA was reduced. The phosphorylation of ERK was decreased in AdOSBP-infected liver and cultured hepatocytes. Importantly, silencing of OSBP in hepatocytes suppressed the induction of SREBP1-c by insulin and resulted in a reduction of TG synthesis. CONCLUSION: Our results demonstrate that OSBP regulates hepatic TG metabolism and suggest the involvement of OSBP in the insulin signaling pathways that control hepatic lipogenesis.
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Yan et al. (2007) studied Hepatic lipogenesis and triglyceride metabolism. Adenovirus-mediated hepatic overexpression of OSBP vs. ORP1L, ORP3, or control was evaluated on Serum lipids, liver tissue triglycerides, and triglyceride secretion rate. Adenovirus-mediated hepatic overexpression of OSBP increased VLDL triglycerides and liver tissue triglycerides in mice, including a 70% increase in the triglyceride secretion rate.
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