May 20, 2006AJP Heart and Circulatory Physiology
Angiotensin II-mediated oxidative stress and procollagen-1 expression in cardiac fibroblasts: blockade by pravastatin and pioglitazone
Key result
The combination of pravastatin and pioglitazone markedly inhibited ANG II-induced oxidative stress, MAPK activation, and procollagen-1 expression in mouse cardiac fibroblasts.
Why the study?
Does the combination of pravastatin and pioglitazone reduce ANG II-mediated oxidative stress and procollagen-1 expression in mouse cardiac fibroblasts?
Population
Mouse cardiac fibroblasts treated with Angiotensin II (ANG II)
Comparison
Combination of pravastatin and pioglitazone vs Pravastatin alone, pioglitazone alone, or no…
Design
Preclinical
Authors
Discussion
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Implication
Should not change clinical practice; hypothesis-generating for combined statin-PPARγ effects on ANG II-driven cardiac fibrosis.
Structured PICO
Does the combination of pravastatin and pioglitazone reduce ANG II-mediated oxidative stress and procollagen-1 expression in mouse cardiac fibroblasts?
P
Population
Mouse cardiac fibroblasts treated with Angiotensin II (ANG II)
I
Intervention
Combination of pravastatin (0.1 microM) and pioglitazone (5 microM)
C
Comparator
Pravastatin alone, pioglitazone alone, or no treatment
O
Outcome
Procollagen-1 expression and oxidative stress (NADPH oxidase expression, superoxide anion production)surrogate
The combination of pravastatin and pioglitazone synergistically inhibits ANG II-mediated oxidative stress and procollagen-1 expression in mouse cardiac fibroblasts, suggesting a potential antifibrotic mechanism.
Cite This Study
Chen et al. (2006) studied ANG II-treated mouse cardiac fibroblasts. Combination of pravastatin and pioglitazone vs. ANG II alone or individual treatments was evaluated on procollagen-1 expression, NADPH oxidase expression, superoxide anion production, and MAPK activation. The combination of pravastatin and pioglitazone markedly inhibited ANG II-induced oxidative stress, MAPK activation, and procollagen-1 expression in mouse cardiac fibroblasts.