Protein-protein interaction of FHL3 with FHL2 and visualization of their interaction by green fluorescent proteins (GFP) two-fusion fluorescence resonance energy transfer (FRET)

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Population

Muscle myoblast C2C12 cells and yeast two-hybrid system

Design

Preclinical

Authors

Discussion

No immediate clinical implications; leaves open functional roles of FHL2-FHL3 mitochondrial interaction in cardiac muscle.

Population

Muscle myoblast C2C12 cells and yeast two-hybrid system

Intervention

Green fluorescent protein (GFP) and blue fluorescent protein (BFP) fusion constructs of FHL2 and FHL3, and site-directed mutagenesis

Outcome

Protein-protein interaction between FHL2 and FHL3 and their subcellular localizationsurrogate

FHL3 is a novel interacting partner of FHL2, and they colocalize in the mitochondria of muscle myoblasts, which can be visualized using FRET.

Li et al. (2000) studied this question.

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