Localization of thioredoxin from Escherichia coli in an osmotically sensitive compartment.

The low molecular weight dithiol protein thioredoxin was released from Escherichia coli strain W3110 grown to mid-log phase b y several nondisruptive treatments.Rapid freezing of cells in media or in a Tris buffer (pH 7.4) containing 3 mM EDTA released greater than 90% of the thioredoxin.Thioredoxin was also quantitatively released b y the osmotic shock procedures described by