What question did this study set out to answer?

To determine the role of the Xenopus BRCT-repeat protein Xmus101 in recruiting the helicase cofactor Cdc45 to origins of DNA replication.

November 18, 2002The Journal of Cell BiologyOpen Access

The Xenopus Xmus101 protein is required for the recruitment of Cdc45 to origins of DNA replication

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Molecular study demonstrates Xmus101 is required for origin loading of Cdc45 in Xenopus, revealing a bifurcated pre-replication pathway.

To determine the role of the Xenopus BRCT-repeat protein Xmus101 in recruiting the helicase cofactor Cdc45 to origins of DNA replication.
Analyzed eukaryotic DNA replication origin assembly and protein interactions using the Xenopus model system.
Investigated the chromatin-binding dependencies among Xmus101, origin recognition complex (ORC), S-Cdk, MCM2-7, and Cdc45.
Xmus101 is essential for loading Cdc45 onto replication origins, a required step for origin unwinding.
Chromatin association of Xmus101 depends strictly on ORC, but occurs independently of both S-Cdk and the MCM2-7 complex.
Replication initiation complex assembly bifurcates after ORC binding into two parallel pathways governed by MCM2-7 and Xmus101 that cooperate to recruit Cdc45.

Hatten et al. (2002) studied this question.

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