Fatty acid methyl esters suitable for gas chromatographic analysis were prepared by treatment of bacterial cells with sodium methoxide (1 N) in anhydrous methanol. The methanolysis of cells, washed or not with sterile water, was carried out at room temperature for 5 min. This method was effective for Gram-positive and Gram-negative bacteria, but not for growing yeast cells. The coefficient of variation of fatty acid methyl ester values was 4–7% of the mean values (range 0.4–25%).
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Rozés et al. (1993) studied this question.
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