What question did this study set out to answer?

To determine why somatic angiotensin-converting enzyme is shed less efficiently than testis ACE and whether this difference is caused by cleavage at distinct stalk sites.

April 25, 2000Biochemical JournalOpen Access

Shedding of somatic angiotensin-converting enzyme (ACE) is inefficient compared with testis ACE despite cleavage at identical stalk sites

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Authors

ZWZenda WoodmanUniversity of Cape Town SOSylvester Y. OppongUniversity of Ghana SCSarah CookLondon School of Hygiene & Tropical Medicine

Discussion

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Implication

Challenges prior assumption of alternative cleavage in somatic ACE; supports motif occlusion hypothesis and leaves open secretase regulation.

Key Points

To determine why somatic angiotensin-converting enzyme is shed less efficiently than testis ACE and whether this difference is caused by cleavage at distinct stalk sites.
Expressed human somatic ACE and testis ACE in transfected Chinese hamster ovary cells to compare release rates and response to phorbol ester activation.
Mapped C-termini of soluble human and porcine somatic ACE using site-specific anti-peptide antibodies, competitive ELISA, and mass spectrometry.
Human somatic ACE was shed substantially less efficiently than testis ACE in Chinese hamster ovary cells and showed minimal shedding response to phorbol ester stimulation.
Antibody binding, competitive ELISA, and mass-spectral sequencing established that soluble somatic ACE is cleaved solely at the Arg(1203)/Ser(1204) peptide bond.
The somatic cleavage site is identical to the Arg(627)/Ser(628) cleavage site in testis ACE, indicating that domain-mediated occlusion rather than alternative cleavage causes inefficient shedding.

Cite This Study

Woodman et al. (2000) studied this question.

synapsesocial.com/papers/6a6f45578031ec7bb1dbace2 https://doi.org/10.1042/bj3470711

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Implication

Challenges prior assumption of alternative cleavage in somatic ACE; supports motif occlusion hypothesis and leaves open secretase regulation.

Shedding of somatic angiotensin-converting enzyme (ACE) is inefficient compared with testis ACE despite cleavage at identical stalk sites

Discussion

Member takes

Implication

Key Points

Cite This Study

Shedding of somatic angiotensin-converting enzyme (ACE) is inefficient compared with testis ACE despite cleavage at identical stalk sites

Discussion

Member takes

Implication

Key Points

Cite This Study

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