What question did this study set out to answer?

To determine whether unstimulated human peripheral blood monocytes undergo necrosis or programmed cell death (apoptosis), and to assess whether inflammatory stimuli promote cell survival.

March 1, 1991The Journal of Immunology

Lipopolysaccharide, tumor necrosis factor-alpha, and IL-1 beta prevent programmed cell death (apoptosis) in human peripheral blood monocytes

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Authors

DMD F ManganWest Virginia University GWG. Rickey WelchAgricultural Research Service SWSharon M. WahlRoche (Switzerland)

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Implication

In vitro study demonstrates that LPS, TNF-alpha, and IL-1 beta prevent apoptosis in human monocytes, suggesting inflammatory stimuli actively sustain immune cell longevity.

Key Points

To determine whether unstimulated human peripheral blood monocytes undergo necrosis or programmed cell death (apoptosis), and to assess whether inflammatory stimuli promote cell survival.
Cultured isolated human peripheral blood monocytes in the absence of serum or cytokines, comparing them to cells killed by nonphysiologic stressors (pH 4.2, 56 °C, or freeze-thaw cycles).
Evaluated apoptotic morphology, nuclear condensation, and genomic DNA fragmentation into ~200 bp multiples.
Treated cultured monocytes with Escherichia coli lipopolysaccharide (concentrations down to 0.1 ng/ml), recombinant human TNF-alpha, or IL-1 beta to assess survival outcomes.
Unstimulated monocytes spontaneously died through programmed cell death, exhibiting nuclear condensation, membrane blebbing, cell shrinkage, and internucleosomal DNA fragmentation.
Nonphysiologic insults rapidly killed monocytes without causing DNA fragmentation, establishing that DNA laddering was specific to internally regulated apoptosis.
Treatment with E. coli LPS (≥0.1 ng/ml), recombinant TNF-alpha, or IL-1 beta prevented DNA fragmentation and substantially prolonged monocyte viability.

Cite This Study

Mangan et al. (1991) studied this question.

synapsesocial.com/papers/6a6f474cb27f15817826ed38 https://doi.org/10.4049/jimmunol.146.5.1541

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Implication

In vitro study demonstrates that LPS, TNF-alpha, and IL-1 beta prevent apoptosis in human monocytes, suggesting inflammatory stimuli actively sustain immune cell longevity.

Lipopolysaccharide, tumor necrosis factor-alpha, and IL-1 beta prevent programmed cell death (apoptosis) in human peripheral blood monocytes

Discussion

Member takes

Implication

Key Points

Cite This Study

Lipopolysaccharide, tumor necrosis factor-alpha, and IL-1 beta prevent programmed cell death (apoptosis) in human peripheral blood monocytes

Discussion

Member takes

Implication

Key Points

Cite This Study

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