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December 1, 1986The Journal of Cell BiologyOpen Access

Titin and myosin, but not desmin, are linked during myofibrillogenesis in postmitotic mononucleated myoblasts.

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Population

Cultured muscle cells

Comparison

Perturbation of myofibrillogenesis with Colcemid… vs Normal myofibrillogenesis (untreated cells)

Design

Preclinical

Authors

CHChristiane HillUniversity of North Carolina at Chapel HillSDSevin Teoman DuranBursa Uludağ Üni̇versi̇tesi̇ZLZ X LinUniversity City Science Center

Discussion

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Implication

No immediate clinical implications for sarcomere disorders; leaves open whether titin-myosin coupling holds in vivo or human disease.

Key Points

  • To examine the temporal expression and spatial distribution of titin, myosin heavy chains, and desmin during myofibril assembly in cultured myoblasts and myotubes under normal and perturbed conditions.
  • Cultured developing myoblasts and multinucleated myotubes were evaluated using monoclonal antibodies specific for titin, myofibrillar myosin heavy chains (MHC), and desmin.
  • Myofibrillogenesis was experimentally perturbed by treating cells with the microtubule-altering agents Colcemid or taxol.
  • Titin and MHC synthesis initiated simultaneously in postmitotic mononucleated myoblasts, with both proteins co-localizing from nonstriated to striated myofibrils, while desmin synthesis began earlier in replicating presumptive myoblasts.
  • Colcemid-treated cells formed rounded myosacs with extensive desmin cables and delayed myofibril striation, yet preserved the characteristic spatial and temporal alignment between titin and MHC.
  • Taxol induced pseudo-striated myofibrils devoid of thin filaments and Z-bands, within which titin maintained its characteristic sarcomeric periodicity alongside thick filaments.

Structured PICO

P
Population
Cultured muscle cells (replicating presumptive myoblasts, postmitotic mononucleated myoblasts, and multinucleated myotubes)
I
Intervention
Perturbation of myofibrillogenesis with Colcemid or taxol
C
Comparator
Normal myofibrillogenesis (untreated cells)
O
Outcome
Temporal and topographical localization of titin, myosin heavy chains (MHC), and desmin during myofibrillogenesissurrogate

Titin and myosin synthesis and spatial deployment are tightly coupled during sarcomere assembly, suggesting interaction between two relatively autonomous organizing centers.

Cite This Study

Hill et al. (1986) studied this question.

synapsesocial.com/papers/6a6f484db27f15817826ed86https://doi.org/10.1083/jcb.103.6.2185
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Selected Topics in Skeletal Myogenesis1973 · 105 citations
  2. 2AN ANALYSIS OF MYOGENESIS BY THE USE OF FLUORESCENT ANTIMYOSIN1957 · 322 citations
  3. 3The relationship between stress fiber-like structures and nascent myofibrils in cultured cardiac myocytes.1984 · 268 citations
  4. 4Identification of an N2 line protein of striated muscle.1980 · 138 citations
  5. 5Effects of colcemid and taxol on microtubules and intermediate filaments in chick embryo fibroblasts1986 · 34 citations