Key result
BMP4 increases canonical transient receptor potential (TRPC) protein expression and basal intracellular Ca2+ in pulmonary arterial smooth muscle cells by activating p38 MAPK and ERK1/2 signaling pathways.
Population
Adult male Wistar rats (300–500 g) used to isolate distal pulmonary arterial smooth muscle cells (PASMCs)
Comparison
Recombinant human BMP4 for up to 60 hours, with… vs Vehicle, dissolvent DMSO, or non-targeting siRNA
Design
Preclinical
Authors
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May inform mechanistic studies of pulmonary hypertension; leaves open translation to human disease or therapy.
p-value: p=<0.05
BMP4 increases intracellular calcium in pulmonary arterial smooth muscle cells by upregulating TRPC channels via p38 and ERK1/2 MAPK signaling pathways, providing mechanistic insight into pulmonary hypertension pathogenesis.
Li et al. (2013) studied Pulmonary Arterial Hypertension. BMP4 vs. Vehicle control was evaluated on TRPC1, TRPC4, and TRPC6 expression and basal intracellular Ca2+ concentration (p=<0.05). BMP4 increases canonical transient receptor potential (TRPC) protein expression and basal intracellular Ca2+ in pulmonary arterial smooth muscle cells by activating p38 MAPK and ERK1/2 signaling pathways.
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