What question did this study set out to answer?

To determine whether ADP-ribosylation factor is required for the binding of the coatomer complex to Golgi membranes during vesicle formation.

June 1, 1993Journal of Biological ChemistryOpen Access

Binding of coatomer to Golgi membranes requires ADP-ribosylation factor

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Authors

DPDavid J. PalmerNorthwestern University JHJ. Bernd HelmsUtrecht University CBC J BeckersUniversity of Würzburg

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Implication

In vitro study demonstrates that myristoylated ADP-ribosylation factor recruits coatomer to Golgi membranes, indicating a sequential mechanism in vesicle coat assembly.

Key Points

To determine whether ADP-ribosylation factor is required for the binding of the coatomer complex to Golgi membranes during vesicle formation.
Monitored coatomer recruitment to Golgi membranes using an antibody targeting the beta-COP subunit.
Tested binding dependence on myristoylation status of ARF, membrane-associated proteins, and non-hydrolyzable GTP analogues including GTP gamma S.
Assessed sequential assembly by pre-incubating isolated membranes with ARF and GTP gamma S prior to re-isolation and beta-COP incubation.
Coatomer binding to Golgi membranes required myristoylated ARF, whereas non-myristoylated ARF failed to support recruitment.
ARF and beta-COP bound stoichiometrically in a saturable manner that required a membrane-associated protein and was enhanced by GTP gamma S.
Membranes pre-incubated with ARF and GTP gamma S supported subsequent beta-COP binding after re-isolation, demonstrating sequential assembly.

Cite This Study

Palmer et al. (1993) studied this question.

synapsesocial.com/papers/6a6f4f1d26770c2b8ddfedbe https://doi.org/10.1016/s0021-9258(19)50311-8

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Implication

In vitro study demonstrates that myristoylated ADP-ribosylation factor recruits coatomer to Golgi membranes, indicating a sequential mechanism in vesicle coat assembly.

Binding of coatomer to Golgi membranes requires ADP-ribosylation factor

Discussion

Member takes

Implication

Key Points

Cite This Study

Binding of coatomer to Golgi membranes requires ADP-ribosylation factor

Discussion

Member takes

Implication

Key Points

Cite This Study

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