Key result
The real-time RT-PCR method using a negative regression model reliably determined FMDV 146S particle concentrations, demonstrating 95.5-99.0% correlation with complement fixation test results.
Effect estimate: correlation 95.5-99.0%
A newly developed rtRT-PCR method allows for rapid and reliable estimation of the 146S antigen concentration in FMD vaccines.
Method for determination of foot-and-mouth disease (FMD) virus 146S component concentration with real-time reverse transcription – polymerase chain reaction (rtRT-PCR) in vaccine raw materials is developed. Negative regression model of С 146S = (30.269 – C t )/4.155 type allowing determination of FMDV 146S particle concentrations based on the amplification threshold cycle values (C t ) is proposed. It has been experimentally proven that the quantity of the 146S component determined by the real-time RT-PCR method using developed regression model and contained in the inoculation dose of FMD vaccine confers protection to the vaccinated animals against generalized FMD of A, O, Asia-1 types. rtRT-PCR method is highly sensitive and allows rapid and highly reliable estimation of the 146S antigen concentration in FMD vaccine. The method for 146S particle quantity determination by real-time RT-PCR using the regression model is reliable and demonstrates high correlation (95.5–99.0%) with the complement fixation test results.
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Доронин et al. (2018) studied Foot-and-mouth disease (FMD). Real-time reverse transcription-polymerase chain reaction (rtRT-PCR) vs. Complement fixation test was evaluated on 146S component concentration determination (correlation 95.5-99.0%). The real-time RT-PCR method using a negative regression model reliably determined FMDV 146S particle concentrations, demonstrating 95.5-99.0% correlation with complement fixation test results.
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