Why the study?
Does the use of multiple diagnostic targets enhance the sensitivity of rRT-PCR for detecting FMDV in clinical samples?
Population
394 RNA samples extracted from suspensions of epithelial tissue obtained from suspect foot-and-mouth disease…
Comparison
Real-time reverse transcription-polymerase chain… vs Real-time RT-PCR assay targeting RNA polymerase
Design
Other
Authors
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Supports multi-target rRT-PCR for FMDV detection; leaves open whether it should replace single-target assays in routine diagnostics.
Does the use of multiple diagnostic targets enhance the sensitivity of rRT-PCR for detecting FMDV in clinical samples?
Using multiple diagnostic targets in rRT-PCR assays can enhance the sensitivity of detecting FMDV by mitigating the impact of nucleotide substitutions in circulating field strains.
King et al. (2006) studied this question.
Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context: