Key result
The multiplexed RT-PCR assay demonstrated a diagnostic sensitivity of 93.9% (95% CI, 89.8-96.4%) for FMDV, compared to 98.1% for two singleplex rRT-PCR assays used in combination.
Why the study?
Does mRT-PCR accurately detect and differentiate FMDV from look-alike disease viruses in livestock samples?
Population
287 field samples, including 247 samples from suspected cases of foot-and-mouth disease collected from 65…
Comparison
Multiplexed reverse transcription-PCR… vs Two singleplex rRT-PCR assays, using virus…
Design
Other
Authors
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May support multiplexed livestock diagnostics; leaves open whether modest sensitivity loss warrants field validation before adoption.
Observational (n=287)
Yes
Does mRT-PCR accurately detect and differentiate FMDV from look-alike disease viruses in livestock samples?
Absolute Event Rate: 93.9% vs 98.1%
The mRT-PCR assay provides high diagnostic sensitivity (93.9%) for FMDV and can simultaneously screen for and differentiate other viruses causing clinically similar diseases in livestock.
Hindson et al. (2008) conducted an observational in Foot-and-mouth disease (n=287). Multiplexed reverse transcription-PCR (mRT-PCR) assay vs. Two singleplex rRT-PCR assays used in combination was evaluated on Diagnostic sensitivity for FMDV. The multiplexed RT-PCR assay demonstrated a diagnostic sensitivity of 93.9% (95% CI, 89.8-96.4%) for FMDV, compared to 98.1% for two singleplex rRT-PCR assays used in combination.
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