December 1, 2000Journal of Clinical MicrobiologyOpen Access

Development of Reverse Transcription-PCR (Oligonucleotide Probing) Enzyme-Linked Immunosorbent Assays for Diagnosis and Preliminary Typing of Foot-and-Mouth Disease: a New System Using Simple and Aqueous-Phase Hybridization

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Population

Foot-and-mouth disease virus (FMDV) RNA genome (all seven serotypes)

Design

Preclinical

Authors

SASøren AlexandersenDeakin University MFM. ForsythThe Pirbright Institute SRScott M. ReidThe Pirbright Institute

Discussion

Member takes

Implication

May aid FMDV research diagnostics in animals; leaves open field validation before veterinary use.

Structured PICO

Population

Foot-and-mouth disease virus (FMDV) RNA genome (all seven serotypes)

Intervention

Reverse transcription-PCR (RT-PCR)-enzyme-linked immunosorbent assay system with a rapid hybridization step using a biotin-labeled internal oligonucleotide

Outcome

Detection and preliminary typing of FMDV

The development of a highly specific and sensitive RT-PCR-ELISA system allows for rapid diagnosis and strain differentiation of foot-and-mouth disease virus.

Cite This Study

Alexandersen et al. (2000) studied this question.

synapsesocial.com/papers/6a7dfda37c193befc433a7c3 https://doi.org/10.1128/jcm.38.12.4604-4613.2000

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Development of Reverse Transcription-PCR (Oligonucleotide Probing) Enzyme-Linked Immunosorbent Assays for Diagnosis and Preliminary Typing of Foot-and-Mouth Disease: a New System Using Simple and Aqueous-Phase Hybridization

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Development of Reverse Transcription-PCR (Oligonucleotide Probing) Enzyme-Linked Immunosorbent Assays for Diagnosis and Preliminary Typing of Foot-and-Mouth Disease: a New System Using Simple and Aqueous-Phase Hybridization

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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Also Consider