Key result
Real-time RT-PCR detected all 204 samples positive by virus isolation and ELISA, plus an additional 60 samples (18%), providing results in less than one day.
Why the study?
Does real-time RT-PCR improve the sensitivity and speed of laboratory diagnosis of foot and mouth disease compared to virus isolation and ELISA in epithelial samples?
Population
334 epithelia samples received from eighteen countries between August 2002 and January 2004
Comparison
Automated real-time reverse transcription… vs Virus isolation in cell culture and antigen…
Authors
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May enhance FMD diagnostic sensitivity and speed; leaves open prospective validation before routine adoption.
Observational (n=334)
No
Does real-time RT-PCR improve the sensitivity and speed of laboratory diagnosis of foot and mouth disease compared to virus isolation and ELISA in epithelial samples?
Real-time RT-PCR provides a more sensitive and rapid method for diagnosing foot and mouth disease compared to traditional virus isolation and ELISA.
Shaw et al. (2004) conducted an observational in Foot and mouth disease (n=334). Automated real-time reverse transcription polymerase chain reaction (RT-PCR) vs. Virus isolation (VI) in cell culture and antigen detection ELISA was evaluated on Detection of FMD virus genome. Real-time RT-PCR detected all 204 samples positive by virus isolation and ELISA, plus an additional 60 samples (18%), providing results in less than one day.
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