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December 1, 2008BMC Veterinary ResearchOpen Access

Diagnosis of foot-and mouth disease by real time reverse transcription polymerase chain reaction under field conditions in Brazil

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Why the study?

Does real-time RT-PCR reliably detect FMDV compared to virus isolation and ELISA in cattle under field conditions?

Population

460 oral swabs from cattle in Brazil (200 from FMD-free areas, 260 from FMD outbreak areas)

Comparison

Real-time reverse transcriptase polymerase chain… vs Virus isolation and enzyme-linked immunosorbent…

Design

Cross-sectional

Authors

TPTatiane A. PaíxãoUniversidade Federal de Minas GeraisANAlcina Vieira Carvalho NetaAutoliv (Sweden)NPNaimes O Paiva

Discussion

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Implication

RT-PCR aligned with outbreak status in this cross-sectional study; leaves open routine field adoption pending prospective validation and infrastructure assessment.

Structured PICO

Does real-time RT-PCR reliably detect FMDV compared to virus isolation and ELISA in cattle under field conditions?

P
Population
460 oral swabs from cattle in Brazil (200 from FMD-free areas, 260 from FMD outbreak areas)
I
Intervention
Real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay
C
Comparator
Virus isolation and enzyme-linked immunosorbent assay (ELISA)
O
Outcome
Detection of foot-and-mouth disease virus (FMDV)surrogate

Real-time RT-PCR is a reliable technique for detecting FMDV in cattle under field conditions, though infrastructure limitations may hinder its routine use.

Limitations

  • Lack of infrastructure in certain areas with high risk of FMD may be a limiting factor for using real-time RT-PCR as a routine diagnostic tool

Cite This Study

Paíxão et al. (2008) studied this question.

synapsesocial.com/papers/6a729dd2439bab0cabc3e2b7https://doi.org/10.1186/1746-6148-4-53
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Enhanced laboratory diagnosis of foot and mouth disease by real-time polymerase chain reaction2004 · 59 citations
  2. 2Detection of Foot-and-Mouth Disease Virus: Comparative Diagnostic Sensitivity of Two Independent Real-Time Reverse Transcription-Polymerase Chain Reaction Assays2006 · 95 citations
  3. 3Evaluation of the portable Cepheid SmartCycler real‐time PCR machine for the rapid diagnosis of foot‐and‐mouth disease2002 · 44 citations
  4. 4Implementation in Australia of molecular diagnostic techniques for the rapid detection of foot and mouth disease virus2004 · 12 citations
  5. 5Detection of foot-and-mouth disease virus from culture and clinical samples by reverse transcription-PCR coupled to restriction enzyme and sequence analysis2003 · 35 citations