Key result
Field-ready rRT-PCR platform correctly identifies ~92% of FMDV serotypes in positive field samples.
Population
57 FMDV-positive samples, 6 non-FMDV vesicular disease samples, and 144 samples collected from pre-clinical…
Comparison
Field-ready real-time PCR platform using a… vs OIE-recommended laboratory-based rRT-PCR
Design
Other
Authors
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Supports field-based FMDV detection in East Africa; leaves open need for prospective validation before routine use.
Observational (n=144)
Yes
A field-ready rRT-PCR platform provides highly sensitive and rapid detection and characterization of foot-and-mouth disease virus in endemic settings.
Howson et al. (2017) conducted an observational in Foot-and-mouth disease (n=144). Field-ready real-time RT-PCR (rRT-PCR) assay on T-COR™ 8 platform vs. OIE-recommended laboratory-based rRT-PCR was evaluated on Correct identification of FMDV serotype. A field-ready rRT-PCR platform correctly identified the serotype of 91.7% (33/36) of FMDV-positive samples and reliably detected FMDV RNA in 144 field samples across East Africa.
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