Key result
Tailored serotype-specific real-time RT-PCR assays correctly identified FMDV serotypes A, O, SAT 1, and SAT 2 in 71 field samples from East Africa.
The newly developed serotype-specific real-time RT-PCR assays can accurately detect and characterize FMDV lineages circulating in East Africa, offering a rapid tool for disease control.
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New FMDV serotype-specific rRT-PCR panel may aid monitoring programs; leaves open large-scale field validation before routine use.
Rapid, reliable and accurate diagnostic methods provide essential support to programmes that monitor and control foot-and-mouth disease (FMD). While pan-specific molecular tests for FMD virus (FMDV) detection are well established and widely used in endemic and FMD-free countries, current serotyping methods mainly rely either on antigen detection ELISAs or nucleotide sequencing approaches. This report describes the development of a panel of serotype-specific real-time RT-PCR assays (rRT-PCR) tailored to detect FMDV lineages currently circulating in East Africa. These assays target sequences within the VP1-coding region that share high intra-lineage identity, but do not cross-react with FMD viruses from other serotypes that circulate in the region. These serotype-specific assays operate with the same thermal profile as the pan-diagnostic tests making it possible to run them in parallel to produce C T values comparable to the pan-diagnostic test detecting the 3D-coding region. These assays were evaluated alongside the established pan-specific molecular test using field samples and virus isolates collected from Tanzania, Kenya and Ethiopia that had been previously characterised by nucleotide sequencing. Samples (n = 71) representing serotype A (topotype AFRICA, lineage G-I), serotype O (topotypes EA-2 and EA-4), serotype SAT 1 (topotype I (NWZ)) and serotype SAT2 (topotype IV) were correctly identified with these rRT-PCR assays. Furthermore, FMDV RNA from samples that did not contain infectious virus could still be serotyped using these assays. These serotype-specific real-time RT-PCR assays can detect and characterise FMDVs currently circulating in East Africa and hence improve disease control in this region.
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Bachanek‐Bankowska et al. (2016) studied Foot-and-mouth disease (n=71). Serotype-specific real-time RT-PCR assays vs. Established pan-specific molecular test was evaluated on Correct identification of FMDV serotypes. Tailored serotype-specific real-time RT-PCR assays correctly identified FMDV serotypes A, O, SAT 1, and SAT 2 in 71 field samples from East Africa.
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