What question did this study set out to answer?

To determine whether the poly(A) tail of the poliovirus genome is genetically coded through RNA transcription or synthesized by non-templated terminal addition.

December 1, 1975Journal of VirologyOpen Access

Replication of picornaviruses. I. Evidence from in vitro RNA synthesis that poly(A) of the poliovirus genome is genetically coded

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Population

Poliovirus-infected HeLa cells (in vitro model)

Comparison

Isolation and use of a crude replication complex… vs Mock-infected cells

Design

Preclinical

Authors

KDKaroline Dorsch‐HäslerSwissmedic YYYoshiaki YogoTokyo Medical and Dental University EWEckard WimmerCalifornia Institute of Technology

Discussion

Member takes

Implication

May refine picornavirus replication models; leaves open relevance to human pathogenesis or vaccines.

Key Points

To determine whether the poly(A) tail of the poliovirus genome is genetically coded through RNA transcription or synthesized by non-templated terminal addition.
Isolated crude membrane-bound replication complexes from poliovirus-infected HeLa cells to synthesize replicative intermediate (RI), replicative form (RF), and single-stranded (SS) viral RNAs in vitro.
Treated partially purified replication complexes with deoxycholate and sedimented them through sucrose gradients to separate the viral replicase from host terminal adenylate transferase activity.
Measured poly(A) chain lengths on in vitro-synthesized viral RNA forms and tested for transferase dependence on manganese ions and primers.
Soluble replication complexes completely separated from host terminal adenylate transferase activity synthesized viral RNA-linked poly(A) with longer chain lengths than crude complexes.
In vitro-synthesized RF and SS RNA contained poly(A) chains of 50 to 70 nucleotides, whereas RI RNA contained poly(A) tails of at least 200 nucleotides.
Transcription of poly(A) corresponds directly with the complementary 5'-terminal poly(U) sequence on poliovirus minus strands, demonstrating templated genetic coding.

Structured PICO

Population

Poliovirus-infected HeLa cells (in vitro model)

Intervention

Isolation and use of a crude replication complex for synthesis of poliovirus RNA in vitro

Comparator

Mock-infected cells (for terminal adenylate transferase activity comparison)

Outcome

Synthesis and characterization of poly(A) in poliovirus RNA (RI, RF, SS RNA)surrogate

The study provides evidence that polyadenylation of poliovirus RNA occurs by transcription, indicating that the poly(A) tail of the picornavirus genome is genetically coded.

Cite This Study

Dorsch‐Häsler et al. (1975) studied this question.

synapsesocial.com/papers/6a6f584a75498292b708bd5a https://doi.org/10.1128/jvi.16.6.1512-1517.1975

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Replication of picornaviruses. I. Evidence from in vitro RNA synthesis that poly(A) of the poliovirus genome is genetically coded

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Replication of picornaviruses. I. Evidence from in vitro RNA synthesis that poly(A) of the poliovirus genome is genetically coded

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider