What question did this study set out to answer?

To measure and characterize the room-temperature fluorescence and quantum yields of purine and pyrimidine nucleic acid bases in neutral aqueous solution.

February 19, 1971Science

Fluorescence of the Purine and Pyrimidine Bases of the Nucleic Acids in Neutral Aqueous Solution at 300°K

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Authors

MDMalcolm DanielsUniversité Paris-Sud WHWilliam W. HauswirthUniversity of North Texas

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Implication

Spectroscopic analysis detects room-temperature fluorescence in nucleic acid bases, revealing very low quantum yields in neutral aqueous solution.

Key Points

To measure and characterize the room-temperature fluorescence and quantum yields of purine and pyrimidine nucleic acid bases in neutral aqueous solution.
Measured fluorescence of adenine, guanine, cytosine, and uracil in neutral aqueous solution at 300°K.
Employed a digital signal accumulation technique to acquire corrected excitation and emission spectra.
Detected room-temperature emission spectra for all four bases excited at their low-energy absorption maxima.
Determined quantum yields to be 2.6 x 10⁻⁴ for adenine, 3.0 x 10⁻⁴ for guanine, 0.8 x 10⁻⁴ for cytosine, and 0.5 x 10⁻⁴ for uracil.

Cite This Study

Daniels et al. (1971) studied this question.

synapsesocial.com/papers/6a6f5a795d37378ac1dc6bdd https://doi.org/10.1126/science.171.3972.675

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Implication

Spectroscopic analysis detects room-temperature fluorescence in nucleic acid bases, revealing very low quantum yields in neutral aqueous solution.

Fluorescence of the Purine and Pyrimidine Bases of the Nucleic Acids in Neutral Aqueous Solution at 300°K

Discussion

Member takes

Implication

Key Points

Cite This Study

Fluorescence of the Purine and Pyrimidine Bases of the Nucleic Acids in Neutral Aqueous Solution at 300°K

Discussion

Member takes

Implication

Key Points

Cite This Study

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