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IN the course of an investigation on glycogen, the solubility of a sample prepared in the usual way by boiling tissue with 60 % KOH was compared with that of a sample prepared from an aqueous extract of tissue dehydrated by alcohol, and it was observed that the former sample reached a stable value very much more rapidly than the latter. It was therefore decided to prepare glycogen from several sources, without boiling with potassium hydroxide and to determine the solubilities of the products obtained. In the course of the work it was found necessary to revise the method employed for estimating glycogen, and, in order to do this, the solubility of glycogen in aqueous alcohol was investigated.
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Margaret Kerly (1930) studied this question.
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