What question did this study set out to answer?

To identify and characterize the cDNA encoding mouse hepatic oxysterol 7α-hydroxylase and evaluate its regulation relative to cholesterol 7α-hydroxylase.

September 1, 1997Journal of Biological ChemistryOpen Access

Identification and Characterization of a Mouse Oxysterol 7α-Hydroxylase cDNA

View Full Paper

Ask AI

Bookmark

Authors

MSMargrit SchwarzBoehringer Ingelheim (United States)ELErik LundUppsala University RLRichard LatheAlzheimer’s Disease Neuroimaging Initiative

Discussion

Member takes

Implication

Laboratory study demonstrates that Cyp7b1 encodes hepatic oxysterol 7α-hydroxylase in mice, indicating a constitutive pathway for bile acid synthesis.

Key Points

To identify and characterize the cDNA encoding mouse hepatic oxysterol 7α-hydroxylase and evaluate its regulation relative to cholesterol 7α-hydroxylase.
Transfected cultured cells with hippocampal-derived Cyp7b1 cDNA to compare enzymatic and pharmacological activity against hepatic oxysterol 7α-hydroxylase.
Profiled Cyp7b1 mRNA, protein levels, and enzymatic activity during postnatal mouse development.
Challenged adult mice with diets enriched with cholesterol, colestipol, or bile acids to measure changes in Cyp7b1 and cholesterol 7α-hydroxylase expression.
Cyp7b1 cDNA expression in cultured cells yielded an enzyme biochemically and pharmacologically identical to hepatic oxysterol 7α-hydroxylase, with expression rising during the third week of life.
Dietary cholesterol and colestipol induced cholesterol 7α-hydroxylase mRNA but caused no measurable change in oxysterol 7α-hydroxylase activity, mRNA, or protein.
Dietary bile acids suppressed cholesterol 7α-hydroxylase mRNA to undetectable levels while causing only a modest reduction in oxysterol 7α-hydroxylase expression.

Cite This Study

Schwarz et al. (1997) studied this question.

synapsesocial.com/papers/6a6f5c31febe604dd7084d63 https://doi.org/10.1074/jbc.272.38.23995

View Full Paper

Ask AI

Bookmark

Implication

Laboratory study demonstrates that Cyp7b1 encodes hepatic oxysterol 7α-hydroxylase in mice, indicating a constitutive pathway for bile acid synthesis.

Identification and Characterization of a Mouse Oxysterol 7α-Hydroxylase cDNA

Discussion

Member takes

Implication

Key Points

Cite This Study

Identification and Characterization of a Mouse Oxysterol 7α-Hydroxylase cDNA

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider