What question did this study set out to answer?

To resolve conflicting findings regarding Fas signaling and determine whether Bcl-2 family proteins regulate apoptosis triggered by physiological Fas ligand.

December 21, 1999Proceedings of the National Academy of SciencesOpen Access

Activation of Fas by FasL induces apoptosis by a mechanism that cannot be blocked by Bcl-2 or Bcl-x L

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Authors

DHDavid C.S. HuangUniversity of Southern California MHMichael HahneCentre National de la Recherche Scientifique MSMichael SchroeterUniversity of Cologne

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Implication

Laboratory study demonstrates that FasL triggers apoptosis via FADD and caspase-8 in mouse cells, indicating Fas signaling operates independently of Bcl-2 family proteins.

Key Points

To resolve conflicting findings regarding Fas signaling and determine whether Bcl-2 family proteins regulate apoptosis triggered by physiological Fas ligand.
Compared apoptotic activity elicited by natural Fas ligand (membrane-bound or aggregated soluble FasL) against anti-Fas antibodies.
Assessed the requirement of FADD/MORT1, caspase-8, Bcl-2, and Bcl-xL in cell lines and primary lymphocytes and hepatocytes from transgenic mice.
Extensive Fas aggregation by membrane-bound or aggregated soluble FasL consistently triggered apoptosis, whereas anti-Fas antibodies functioned unpredictably as death agonists or antagonists.
Fas-mediated apoptotic signaling strictly required the adaptor protein FADD/MORT1 and caspase-8.
Overexpression of Bcl-2 or Bcl-xL failed to inhibit FasL-induced apoptosis in lymphocytes or hepatocytes, demonstrating distinct pathways.

Cite This Study

Huang et al. (1999) studied this question.

synapsesocial.com/papers/6a6f5c67ac440176ef280c0e https://doi.org/10.1073/pnas.96.26.14871

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Implication

Laboratory study demonstrates that FasL triggers apoptosis via FADD and caspase-8 in mouse cells, indicating Fas signaling operates independently of Bcl-2 family proteins.

Activation of Fas by FasL induces apoptosis by a mechanism that cannot be blocked by Bcl-2 or Bcl-x L

Discussion

Member takes

Implication

Key Points

Cite This Study

Activation of Fas by FasL induces apoptosis by a mechanism that cannot be blocked by Bcl-2 or Bcl-x L

Discussion

Member takes

Implication

Key Points

Cite This Study

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