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July 1, 1999Arthritis & Rheumatism

Freshly isolated cells from RA synovial tissues more strongly expressed mRNA for both IL-7 and IL-15 compared with cells from osteoarthritis tissues, and fibroblast-like synoviocytes produced IL-15 and IL-7 which stimulated T cell proliferation.

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Population

Fibroblast-like synoviocytes, synovial tissue cells, and T cells from patients with rheumatoid arthritis and…

Comparison

In vitro stimulation with IL-1, tumor necrosis… vs Cells from osteoarthritis tissues; unstimulated…

Design

Preclinical

Authors

SHSeishi HaradaMYMasahiro YamamuraHOHideyuki Okamoto

Discussion

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Overview

IL-15/IL-7 from RA synoviocytes may promote synovial T-cell expansion; leaves open their role as therapeutic targets pending clinical validation.

Key Points

  • To examine the production of interleukin-7 (IL-7) and interleukin-15 (IL-15) by rheumatoid arthritis fibroblast-like synoviocytes and evaluate their capacity to stimulate synovial T-cell proliferation.
  • Assessed mRNA and protein levels of IL-7 and IL-15 in synovial tissue cells and fibroblast cell lines using RT-PCR and ELISA.
  • Isolated T cell-enriched populations from rheumatoid arthritis synovial tissues and measured their proliferative response to IL-7, IL-15, and IL-2 using 3H-thymidine incorporation.
  • Rheumatoid arthritis synovial tissue cells exhibited higher mRNA and spontaneous protein release of IL-7 and IL-15 than osteoarthritis tissue cells.
  • Cultured rheumatoid arthritis fibroblast cell lines produced large amounts of IL-15 and smaller amounts of IL-7, with synthesis significantly increased upon stimulation by IL-1 and TNF-alpha.
  • Both IL-7 and IL-15 stimulated the proliferation of synovial T cells, with IL-15 displaying potency similar to IL-2, while IL-7 was comparatively less potent.

Structured PICO

P
Population
Fibroblast-like synoviocytes, synovial tissue cells, and T cells from patients with rheumatoid arthritis (RA) and osteoarthritis (OA)
I
Intervention
In vitro stimulation with IL-1, tumor necrosis factor alpha, IL-7, IL-15, or IL-2
C
Comparator
Cells from osteoarthritis tissues; unstimulated cells
O
Outcome
Messenger RNA (mRNA) and protein levels of IL-7 and IL-15; T cell proliferation (measured by 3H-thymidine incorporation)surrogate

Fibroblast-like synoviocytes in rheumatoid arthritis joints are an important source of IL-15 and IL-7, which may drive local T cell activation and expansion.

Cite This Study

Harada et al. (1999) studied this question.

synapsesocial.com/papers/6a6f5cbfa528af2d65c2f3c5https://doi.org/10.1002/1529-0131(199907)42:7<1508::aid-anr26>3.0.co;2-l
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Differential expression of pro-inflammatory cytokines IL-15Ralpha, IL-15, IL-6 and TNFalpha in synovial fluid from Rheumatoid arthritis patients2015 · 54 citations
  2. 2INCREASED EXPRESSION OF IL-15 IN THE SYNOVIUM OF PATIENTS WITH RHEUMATOID ARTHRITIS COMPARED WITH PATIENTS WITHYERSINIA-INDUCED ARTHRITIS AND OSTEOARTHRITIS1997 · 112 citations
  3. 3Interleukin-16, produced by synovial fibroblasts, mediates chemoattraction for CD4+ T lymphocytes in rheumatoid arthritis1998 · 116 citations
  4. 4Inhibition of the production of proinflammatory cytokines and immunoglobulins by interleukin‐4 in an ex vivo model of rheumatoid synovitis1992 · 185 citations
  5. 5Synovial tissue macrophage as a source of the chemotactic cytokine IL-81991 · 302 citations