Key result
The EV71 subgenogroup B5 strain B5-203P-2013-P10.1 achieved a titer of 10^8 TCID50/ml in Vero cells and elicited cross-neutralizing antibody responses against B5, C4, and C5 strains in mice.
Why the study?
The study was conducted to select candidate strains from Vietnam for the development of a hand, foot and mouth disease vaccine against enterovirus 71.
The EV71 subgenogroup B5 strain B5-203P-2013-P10.1 is a viable candidate for an HFMD vaccine in Vietnam due to its high titer, genetic stability, and cross-neutralizing capability.
Identified Vietnamese EV71 strains may guide local HFMD vaccine candidates; leaves open clinical validation and efficacy trials.
The study aimed to select candidate strains from Vietnam for the development of the hand, foot and mouth disease (HFMD) vaccine against enterovirus 71 (EV71). Annual EV71 virus surveillance data of Pasteur Institute in Ho Chi Minh city from 2003 to 2013 was used to predict evolutionary trends. One hundred VP1 sequences of Vietnam strains from the GenBank database were used to evaluate the identity of acid amin sequences and identify the consensus sequences at key epitopes. As a result, the subgenogroup B5 strain named B5-203P-2013 was selected. The strain was adapted on Vero cells by multiple passages and plaque isolated. The strain B5-203P-2013-P10.1 was selected as a candidate strain for the HFMD vaccine due to possessing genetic stability, hightiter of 108 TCID50/ml and cross-neutralising antibody responses against other subgenogroups B5, C4, C5.
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Nguyen et al. (2022) studied Hand, foot and mouth disease (EV71) (n=5). EV71 candidate strain B5-203P-2013-P10.1 was evaluated on Viral titer and cross-neutralizing antibody response. The EV71 subgenogroup B5 strain B5-203P-2013-P10.1 achieved a titer of 10^8 TCID50/ml in Vero cells and elicited cross-neutralizing antibody responses against B5, C4, and C5 strains in mice.
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