March 1, 1994AJP Endocrinology and Metabolism

Glucose transport and phosphorylation in single cardiac myocytes: rate-limiting steps in glucose metabolism

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Population

Single myocytes isolated from adult rat ventricles

Comparison

Incubation with insulin vs Incubation without insulin

Design

Preclinical

Authors

JMJill K. ManchesterMonsanto (United States)XKXianming KongLiaoning Shihua University JNJeanne M. NerbonneElectrophysiology

Discussion

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Implication

Hypothesis-generating in rat myocytes; leaves open whether phosphorylation limits insulin-stimulated glucose uptake in human heart.

Structured PICO

Population

Single myocytes isolated from adult rat ventricles

Intervention

Incubation with insulin (with either glucose or 2-deoxyglucose)

Comparator

Incubation without insulin

Outcome

Intracellular levels of 2-deoxyglucose-6-phosphate (2-DG-6-P) or glucose and glucose 6-phosphate (G-6-P)surrogate

In adult rat ventricular myocytes, insulin dramatically increases glucose transport such that glucose phosphorylation becomes the rate-limiting step in glucose utilization.

Cite This Study

Manchester et al. (1994) studied this question.

synapsesocial.com/papers/6a6f60c32163a0a01bc3adfb https://doi.org/10.1152/ajpendo.1994.266.3.e326

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Glucose transport and phosphorylation in single cardiac myocytes: rate-limiting steps in glucose metabolism

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Glucose transport and phosphorylation in single cardiac myocytes: rate-limiting steps in glucose metabolism

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider