Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
October 1, 1982The Journal of ImmunologyOpen Access

Phenotypic characteristics of cell lines requiring interleukin 3 for growth.

View Full Paper
Ask AI
Bookmark
Share

Authors

JIJ N IhleUniversity of IowaJKJR KellerNational Institutes of HealthJGJoel S. GreenbergerUniversity of Pittsburgh

Discussion

Loading...

Member takes

Implication

In vitro experimental study demonstrates interleukin 3 dependency and phenotypic diversity in bone marrow-derived cell lines, highlighting distinct hematopoietic lineages.

Key Points

  • Determine whether bone marrow-derived cell lines requiring WEHI-3 conditioned media are specifically dependent on interleukin 3 (IL 3) for growth and characterize their phenotypic profiles.
  • Fractionated WEHI-3 conditioned media using Sephadex G-100, DEAE cellulose, CM cellulose column chromatography, preparative isoelectric focusing, and reverse-phase HPLC.
  • Assayed peak chromatographic fractions concurrently for cell growth-supporting activity and induction of 20 alpha-hydroxysteroid dehydrogenase (20 alpha SDH).
  • Characterized cell surface marker phenotypes across different dependent cell lines (including C3HSFFV and FDC-P1) via immunophenotyping.
  • The growth-promoting activity copurified identically with IL 3 activity across all chromatographic and electrofocusing separation methods, showing shared peak biological activity.
  • All examined WEHI-3 CM-dependent cell lines exhibited readily detectable levels of 20 alpha SDH enzyme activity.
  • Cell surface marker profiles varied substantially between lines: C3HSFFV lacked conventional lymphoid markers except Lyt-1, whereas FDC-P1 expressed Thy-1, Ly-5, and H-11, with other lines showing intermediate phenotypes.

Cite This Study

Ihle et al. (1982) studied this question.

synapsesocial.com/papers/6a6f6f72c2d7c3090826c449https://doi.org/10.4049/jimmunol.129.4.1377
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Regulation of Expression of the Interleukin-2 Receptor on Hematopoietic Cells by Interleukin-31986 · 57 citations
  2. 2Cloning and nucleotide sequence of the murine interleukin‐3 gene1985 · 61 citations
  3. 3Identification of interleukin-6 as an autocrine growth factor for Epstein-Barr virus-immortalized B cells1990 · 206 citations
  4. 4Interleukin-9 (IL-9) stimulates the proliferation of human myeloid leukemic cells1996
  5. 5Differential activity of dexamethasone on IL-2-, IL-4-, or IL-9-induced proliferation of murine factor-dependent T cell lines1996 · 25 citations