Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
June 1, 1993Proceedings of the National Academy of SciencesOpen Access

Mutation of Asp74 of the rat angiotensin II receptor confers changes in antagonist affinities and abolishes G-protein coupling.

View Full Paper
Ask AI
Bookmark
Share

Population

CHO cells expressing wild-type or mutated rat vascular angiotensin II receptor subtype 1

Comparison

Mutation of Asp74 to asparagine or glutamic acid vs Wild-type AT1a receptor

Design

Preclinical

Authors

CBClaire BihoreauInsermCMCatherine MonnotInsermEDEleanor DaviesIQVIA (United Kingdom)

Discussion

Loading...

Member takes

Overview

No clinical translation for AT1 blockade; leaves open Asp74 as a target for receptor modeling studies.

Structured PICO

P
Population
CHO cells expressing wild-type or mutated (Asp74 replaced by Asn or Glu) rat vascular angiotensin II receptor subtype 1 (AT1a)
I
Intervention
Mutation of Asp74 to asparagine (Asn) or glutamic acid (Glu)
C
Comparator
Wild-type AT1a receptor
O
Outcome
Agonist and antagonist binding affinities and transmembrane signaling (inositol phosphate production, cytosolic Ca2+ concentration, DNA synthesis)surrogate

The conserved Asp74 residue of the AT1a receptor is essential for antagonist binding and transmembrane signaling.

Cite This Study

Bihoreau et al. (1993) studied this question.

synapsesocial.com/papers/6a6f779a75498292b708dc0fhttps://doi.org/10.1073/pnas.90.11.5133
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Cloning, characterization, and expression of two angiotensin receptor (AT-1) isoforms from the mouse genome1992 · 312 citations
  2. 2Angiotensin II and alpha-adrenergic agonists inhibit rat liver adenylate cyclase.1981 · 186 citations
  3. 3Cytoplasmic dot hybridization. Simple analysis of relative mRNA levels in multiple small cell or tissue samples.1982 · 1,310 citations
  4. 4MODEL SYSTEMS FOR THE STUDY OF SEVEN-TRANSMEMBRANE-SEGMENT RECEPTORS1991 · 1,369 citations
  5. 5Eleven amino acids (Lys-201 to Lys-211) and 9 amino acids (Gly-222 to Leu-230) in the human thyrotropin receptor are involved in ligand binding1991 · 67 citations