Hyperglycemia Alters the Structure and Hemodynamics of the Developing Embryonic Heart

Figures

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  Figure 1. Hyperglycemia can lead to asymmetric endocardial outflow tract (OFT) cushions visible in the displaced lumen of cross-sectional images. Cross-sectional optical coherence tomography (OCT) images of the OFT showing a vehicle control (A), a vehicle control cross section labelled for reference (B), an osmotic control (C), a d-glucose-treated embryo with the lumen displaced towards the ventral portion of the OFT (D). Scale bar = 70 µm.

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  Figure 2. Hyperglycemia induces asymmetric endocardial OFT cushions at Hamburger–Hamilton stage 24 (HH24). Representative confocal images of a slice of the OFT cushions taken in the sagittal plane cushions labeled, and asterisks marking the two endocardial cushions from a representative vehicle control (A), an osmotic control (B), and a d-glucose-treated embryo (C). Scale bar = 100 µm, equivalent across images.

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  Figure 3. Segmented HH24 OFTs indicate endocardial OFT cushion asymmetry in response to hyperglycemia. Representative, reconstructed microcomputed tomography (Micro-CT) images of the OFT (transparent purple) with the cushions, left inferior, right superior, (purple) identified for a vehicle control (A), an osmotic control (B), and a d-glucose-treated embryo (C). (D) Average percentage difference in OFT cushion volume. Scale bar = 100 µm, equivalent across images. * p < 0.05 ANOVA.

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  Figure 4. Micro-CT analysis of HH24 hearts reveals differences in endocardial OFT properties in response to hyperglycemia. Representative, reconstructed micro-CT images of the atria (white), ventricle (dark purple), and OFT (light purple) for a vehicle control (A), an osmotic control (B), and a d-glucose-treated embryo (C). The average surface area (D) and volume (E) for the heart sections. Scale bar = 100 µm. * p < 0.05 ANOVA.

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  Figure 5. Hyperglycemic conditions can result in changes in the torsion of the OFT. 3D centerlines of a HH24 representative (A) vehicle control embryo and (B) d-glucose-treated embryo to quantify the amount of torsion of the OFT. (C–E) Graphs, separated by treatment, of the average cumulative torsion, with the dashed lines representing + and −1 standard deviations of the OFT torsion plotted against the cumulative arc length. (F) The average cumulative torsion of the three treatments plotted against the average arc length.

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  Figure 6. Hyperglycemia alters the hemodynamics in chick embryonic OFT. OCT image of the longitudinal cross section of a vehicle control HH18 OFT with lumen and myocardium labelled (A). The outer walls and centerline of the OFT identified with the custom MATLAB program. The calculated centerline velocity of the vehicle control (B), osmotic control (C), and d-glucose-treated embryos (D) from the Doppler OCT images. The average velocities (E) for the treatments. Scale bar = 70 µm. * p < 0.05 ANOVA.