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An improved method utilizing J-multiplication for the measurement of 3J(HN, Hα) coupling constant is introduced. The current method benefits from slower relaxation rate of heteronuclear multiple-quantum coherence. Furthermore, it combines incrementation periods for J-multiplication and 15N chemical-shift evolution. This reduces the length of the overall pulse sequence by t1. This, together with favourable relaxation properties of multiple-quantum coherence, leads to narrower linewidths in the F1 dimension relative to the previously presented sensitivity-enhanced, J-multiplied HSQC experiment. The method is demonstrated on human ubiquitin.
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Permi et al. (1999) studied this question.
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