The composition of distilled lime oil has been investigated. The analytical techniques used to fractionate the oils and identify each component were: open column chromatography, gas chromatography, gas chromatography–mass spectrometry. Among the 113 compounds identified, 28 are reported to be present in distilled lime oil for the first time. The main differences between the cold-pressed and the distilled lime oil are also discussed.
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Dugo et al. (1998) studied this question.