What question did this study set out to answer?

To optimize extraction protocols for an abundant protein from undisturbed soils and assess its similarity to arbuscular mycorrhizal fungal hyphal protein and its relationship with water-stable soil aggregates.

September 1, 1996Soil Science

Extraction of an Abundant and Unusual Protein From Soil and Comparison With Hyphal Protein of Arbuscular Mycorrhizal Fungi

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Authors

SWSara F. WrightAgricultural Research Service AUAbha UpadhyayaAgricultural Research Service

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Implication

Comparative laboratory analysis reveals an abundant mycorrhizal fungal protein in acidic soils, indicating a key role in soil aggregate stabilization.

Key Points

To optimize extraction protocols for an abundant protein from undisturbed soils and assess its similarity to arbuscular mycorrhizal fungal hyphal protein and its relationship with water-stable soil aggregates.
Sampled the A-horizons of 12 acidic soils from undisturbed sites across the mid-Atlantic United States.
Extracted soil protein using autoclaved sodium citrate buffers (varying pH from 7.0 to 8.0 and durations from 30 to 90 min at 121°C), quantifying yields using the Bradford assay.
Compared soil extracts to fungal hyphal proteins using SDS-PAGE electrophoresis and indirect ELISA with a monoclonal antibody.
Optimal extraction (50 mM citrate, pH 8.0, 90 min at 121°C) yielded 4.4 to 14.4 mg protein/g of soil; crude soil extracts matched hyphal protein banding patterns on SDS-PAGE and exhibited 60% to 107% of hyphal ELISA reactivity.
Total soil protein concentration was strongly and linearly correlated with soil organic carbon (r² = 0.85, P ≤ 0.001).
The percentage of soil dry weight in 1 to 2-mm water-stable aggregates was positively correlated with ELISA protein values and silt content (P ≤ 0.05), and negatively correlated with sand.

Cite This Study

Wright et al. (1996) studied this question.

synapsesocial.com/papers/6a6f81d4439bab0cabc2d233 https://doi.org/10.1097/00010694-199609000-00003

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Implication

Comparative laboratory analysis reveals an abundant mycorrhizal fungal protein in acidic soils, indicating a key role in soil aggregate stabilization.

Extraction of an Abundant and Unusual Protein From Soil and Comparison With Hyphal Protein of Arbuscular Mycorrhizal Fungi

Discussion

Member takes

Implication

Key Points

Cite This Study

Extraction of an Abundant and Unusual Protein From Soil and Comparison With Hyphal Protein of Arbuscular Mycorrhizal Fungi

Discussion

Member takes

Implication

Key Points

Cite This Study

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