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The reaction of leghaemoglobin (Lb) with oxidized 3,3'-diaminobenzidine has been used to demonstrate the localization of this haemoprotein within the membrane envelopes surrounding the bacteroids in soybean root nodule cells. In fresh tissue sections mounted in a modified Honda medium, the reagent stained the envelope contents a brick-red colour.
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Bergersen et al. (1973) studied this question.
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